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KMID : 1098420080160060446
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Korean Journal of Medicinal Crop Science
2008 Volume.16 No. 6 p.446 ~ p.454
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Extraction and Preprocessing Methods for Ginsenosides Analysis of Panax ginseng C.A. Mayer
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Kim Geum-Soog
Park Chung-Berm
Lee Sung-Woo
Hyun Dong-Yun
Cha Seon-Woo
Kim Young-Ock
Lee Min-Jeong
Song Kyung-Sik
Park Ho-Ki
Kim Young-Chang
Lee Seung-Eun
Son Yeong-Deck
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Abstract
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An advanced extraction method by ultrasonic extraction with applied solid phase extraction (SPE) has been developed for the determination of simultaneous eight major ginsenosides, namely ginsenosides Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, and Rd in the root of Panax ginseng. Four extraction methods including n-BuOH reflux extraction (Method A), 70% EtOH reflux extraction (Method B), 50% MeOH reflux extraction with SPE (Method C), and 50% MeOH ultrasonication with SPE clean-up process (Method D) were investigated for the determination of eight major ginsenosides. Total contents of ginsenosides were highest by extraction of Method C as 2.408±0.011%. However, Method D was evaluated as relatively simpler and more efficient method due to short extraction time, small solvent consumption and less expensive, compared to conservative reflux method. Ginsenosides were also satisfactorily separated with good resolution and the accuracy range was between 1.05 and 4.06% as relative standard deviation (RSD) by Method D. SPE condition and HPLC condition were further optimized for determination of eight major ginsenosides by the ultrasonic extraction method. Conclusively, ultrasonic extraction of 2 g sample of ginseng using ultrasonic bath and 1 loading for SPE was evaluated as proper condition for extraction of ginseng.
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KEYWORD
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Panax ginseng, Ginsenoside, Extraction Method, Ultrasonic extraction
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